RESEARCH AND COLLABORATION
Intrepid Nepal works exclusively with the Center for Molecular Dynamics-Nepal (CMDN) to provide hands on training on the following areas. Medical Students, Biotechnology majors and anyone who is interested in getting into applied Biotechnology can take advantage of our comprehensive training.
REAL TIME PCR (QRT-PCR)
Real-time polymerase chain reaction or quantitative real time polymerase chain reaction (QRT-PCR), not to be confused with RT-PCR (reverse transcriptase PCR), can simultaneously quantify and amplify a specific part of a given DNA molecule. This technique avoids a number of steps that is normally part of regular PCR and thereby not only prevents human error but also minimizes errors in quantification. It is the “real-time” version of quantitative polymerase chain reaction (Q-PCR), itself a modification of polymerase chain reaction. At CMDN, this technique is our primary method of emerging disease monitoring and quantification.
ELISA and WESTERN BLOTTING
The Enzyme-Linked ImmunoSorbent Assay, or ELISA, is used as the standard biochemical technique in identifying a large number of infectious diseases. We have trained expertise in ELISA and other immunological techniques such as SDS PAGE and Western Blotting. With these techniques we can not only detect viral, bacterial and parasitic antigens but also cytokines profiles of various immune cells of the body that may at any time show indications of elevated immune response to a pathogen. We also have experts in applied sciences: microbiology, epidemiology, and bioinformatics; we have medical doctors in our team; we also have an excellent internet service team. We are able to provide training sessions in all of these areas subject to interest and demand.
TRAINING SESSION AVAILABLE. PLEASE INQUIRE.